› 2a40

X-ray diffraction
1.8Å resolution

Ternary complex of the WH2 domain of WAVE with Actin-DNAse I

Released:
DOI: 10.2210/pdb2a40/pdb
PDB entry 2a40 coloured by chain, front view.
PDB entry 2a40 coloured by chain, side view.
PDB entry 2a40 coloured by chain, top view.
Source organisms:
Primary publication:
Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly.
Chereau D, Kerff F, Graceffa P, Grabarek Z, Langsetmo K, Dominguez R
Proc Natl Acad Sci U S A 102 16644-9 (2005)
PMID: 16275905

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-132784 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Actin, alpha skeletal muscle Chains: A, D
Molecule details ›
Chains: A, D
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
Actin-binding protein WASF2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 32 amino acids
Theoretical weight: 3.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y6W5 (Residues: 433-464; Coverage: 6%)
Gene names: WASF2, WAVE2
Sequence domains: WH2 motif

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 4 x CA
Ligand ATP 2 x ATP
Ligand GOL 2 x GOL
Ligand MG 2 x MG
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 118.719Å b: 41.598Å c: 153.048Å
α: 90° β: 108.79° γ: 90°
R-values:
R R work R free
0.169 0.166 0.215
Expression system: Not provided

Quick links

Citations

37 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
36 more

16 mentions without citation

Tools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model.
Kim et al. (2017)
15 more