› 2a6k

X-ray diffraction
3Å resolution

Crystal Structure Analysis of the germline antibody 36-65 Fab in complex with the dodecapeptide SLGDNLTNHNLR

Released:
DOI: 10.2210/pdb2a6k/pdb
PDB entry 2a6k coloured by chain, front view.
PDB entry 2a6k coloured by chain, side view.
PDB entry 2a6k coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Differential epitope positioning within the germline antibody paratope enhances promiscuity in the primary immune response.
Sethi DK, Agarwal A, Manivel V, Rao KV, Salunke DM
Immunity 24 429-38 (2006)
PMID: 16618601

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211592 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Germline antibody 36-65 Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.71 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, H
Molecule details ›
Chains: B, H
Length: 222 amino acids
Theoretical weight: 23.94 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q6PF95 (Residues: 20-116, 117-118, 119-234; Coverage: 48%)
Gene name: Igh
Sequence domains:
Structure domains: Immunoglobulins
DODECAPEPTIDE: SLGDNLTNHNLR Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.36 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 53.135Å b: 144.92Å c: 70.991Å
α: 90° β: 104.35° γ: 90°
R-values:
R R work R free
0.246 0.245 0.264

Quick links

Citations

15 review citations

Protein promiscuity and its implications for biotechnology.
Nobeli et al. (2009)
14 more

3 mentions without citation

Detect, correct, retract: How to manage incorrect structural models.
Wlodawer et al. (2018)
2 more