› 2adf

X-ray diffraction
1.9Å resolution

Crystal Structure and Paratope Determination of 82D6A3, an Antithrombotic Antibody Directed Against the von Willebrand factor A3-Domain

Released:
DOI: 10.2210/pdb2adf/pdb
PDB entry 2adf coloured by chain, front view.
PDB entry 2adf coloured by chain, side view.
PDB entry 2adf coloured by chain, top view.
Source organisms:
Primary publication:
Paratope determination of the antithrombotic antibody 82D6A3 based on the crystal structure of its complex with the von Willebrand factor A3-domain.
Staelens S, Hadders MA, Vauterin S, Platteau C, De Maeyer M, Vanhoorelbeke K, Huizinga EG, Deckmyn H
J Biol Chem 281 2225-31 (2006)
PMID: 16314412

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-233190 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 20.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04275 (Residues: 1683-1874; Coverage: 7%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Ig heavy chain Mem5 Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P84751 (Residues: 1-220; Coverage: 92%)
Sequence domains:
Structure domains: Immunoglobulins
82D6A3 IgG Chain: L
Molecule details ›
Chain: L
Length: 209 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ACY 1 x ACY
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 72.179Å b: 89.076Å c: 123.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.22
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

The Role of von Willebrand Factor in Vascular Inflammation: From Pathogenesis to Targeted Therapy.
Gragnano et al. (2017)
2 more

17 mentions without citation

Von Willebrand factor: A key glycoprotein involved in thrombo-inflammatory complications of COVID-19.
Choudhary et al. (2021)
16 more