› 2ady

X-ray diffraction
2.5Å resolution

Structural Basis of DNA Recognition by p53 Tetramers (complex IV)

Released:
DOI: 10.2210/pdb2ady/pdb
PDB entry 2ady coloured by chain, front view.
PDB entry 2ady coloured by chain, side view.
PDB entry 2ady coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of DNA recognition by p53 tetramers.
Kitayner M, Rozenberg H, Kessler N, Rabinovich D, Shaulov L, Haran TE, Shakked Z
Mol Cell 22 741-753 (2006)
PMID: 16793544

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115068 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
5'-D(*CP*GP*GP*AP*CP*AP*TP*GP*TP*CP*CP*G)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 91.997Å b: 67.903Å c: 74.989Å
α: 90° β: 92.97° γ: 90°
R-values:
R R work R free
0.169 0.164 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

51 review citations

Blinded by the Light: The Growing Complexity of p53.
Vousden et al. (2009)
50 more

8 mentions without citation

Absence of a simple code: how transcription factors read the genome.
Slattery et al. (2014)
7 more