› 2ag9

X-ray diffraction
2.2Å resolution

Crystal Structure of the Y137S mutant of GM2-Activator Protein

Released:
DOI: 10.2210/pdb2ag9/pdb
PDB entry 2ag9 coloured by chain, front view.
PDB entry 2ag9 coloured by chain, side view.
PDB entry 2ag9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure analysis of phosphatidylcholine-GM2-activator product complexes: evidence for hydrolase activity.
Wright CS, Mi LZ, Lee S, Rastinejad F
Biochemistry 44 13510-21 (2005)
PMID: 16216074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ganglioside GM2 activator Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17900 (Residues: 32-193; Coverage: 95%)
Gene name: GM2A
Sequence domains: ML domain
Structure domains: Ganglioside GM2 activator

Ligands and Environments

2 bound ligands:
Ligand IPA 14 x IPA
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 41.49Å b: 48.39Å c: 55.59Å
α: 76.16° β: 87.7° γ: 87.54°
R-values:
R R work R free
0.243 0.243 0.334
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Lipid transfer proteins: the lipid commute via shuttles, bridges and tubes.
Wong et al. (2019)
6 more

2 mentions without citation

Drug discovery using chemical systems biology: identification of the protein-ligand binding network to explain the side effects of CETP inhibitors.
Xie et al. (2009)
1 more