› 2alz

X-ray diffraction
2.5Å resolution

Ternary Complex of hPoli with DNA and dCTP

Released:
DOI: 10.2210/pdb2alz/pdb
PDB entry 2alz coloured by chain, front view.
PDB entry 2alz coloured by chain, side view.
PDB entry 2alz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair.
Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK
Structure 13 1569-77 (2005)
PMID: 16216587

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115076 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 44.04 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UNA4 (Residues: 50-439; Coverage: 53%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
5'-D(*TP*GP*GP*GP*GP*TP*CP*CP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*GP*GP*AP*CP*CP*(DOC))-3' Chain: P
Molecule details ›
Chain: P
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand DCP 1 x DCP
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6522
Unit cell:
a: 98.114Å b: 98.114Å c: 202.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.272
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

10 review citations

Eukaryotic translesion polymerases and their roles and regulation in DNA damage tolerance.
Waters et al. (2009)
9 more

17 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
16 more