› 2av7

X-ray diffraction
2.05Å resolution

Crystal structure of HTLV-1 TAX peptide Bound to Human Class I MHC HLA-A2 with the K66A mutation in the heavy chain.

Released:
DOI: 10.2210/pdb2av7/pdb
PDB entry 2av7 coloured by chain, front view.
PDB entry 2av7 coloured by chain, side view.
PDB entry 2av7 coloured by chain, top view.
Source organisms:
Primary publication:
Unraveling a hotspot for TCR recognition on HLA-A2: evidence against the existence of peptide-independent TCR binding determinants.
Gagnon SJ, Borbulevych OY, Davis-Harrison RL, Baxter TK, Clemens JR, Armstrong KM, Turner RV, Damirjian M, Biddison WE, Baker BM
J Mol Biol 353 556-73 (2005)
PMID: 16197958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137667 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 275 amino acids
Theoretical weight: 31.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Tax-1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell lymphotrophic virus type 1 (Caribbean isolate)
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax

Ligands and Environments

1 bound ligand:
Ligand GOL 20 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 50.32Å b: 62.532Å c: 74.801Å
α: 82.13° β: 76.57° γ: 78.06°
R-values:
R R work R free
0.175 0.171 0.234
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Conformational changes and flexibility in T-cell receptor recognition of peptide-MHC complexes.
Armstrong et al. (2008)
8 more

1 mention without citation

Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope.
Song et al. (2017)