› 2b3y

X-ray diffraction
1.85Å resolution

Structure of a monoclinic crystal form of human cytosolic aconitase (IRP1)

Released:
DOI: 10.2210/pdb2b3y/pdb
PDB entry 2b3y coloured by chain, front view.
PDB entry 2b3y coloured by chain, side view.
PDB entry 2b3y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human iron regulatory protein 1 as cytosolic aconitase.
Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC
Structure 14 129-39 (2006)
PMID: 16407072

Function and Biology Details

Reaction catalysed: 
(1a) citrate = cis-aconitate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149229 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytoplasmic aconitate hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 888 amino acids
Theoretical weight: 98.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21399 (Residues: 2-889; Coverage: 100%)
Gene names: ACO1, IREB1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ACT 3 x ACT
Ligand SF4 2 x SF4
Ligand GOL 2 x GOL
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 63.91Å b: 215.47Å c: 64.01Å
α: 90° β: 72° γ: 90°
R-values:
R R work R free
0.213 0.211 0.261
Expression system: Escherichia coli

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Citations

36 review citations

The role of iron regulatory proteins in mammalian iron homeostasis and disease.
Rouault TA. (2006)
35 more

12 mentions without citation

Metabolic Enzymes Enjoying New Partnerships as RNA-Binding Proteins.
Castello et al. (2015)
11 more