› 2b4b

X-ray diffraction
2Å resolution

SSAT+COA+BE-3-3-3, K26R mutant

Released:
DOI: 10.2210/pdb2b4b/pdb
PDB entry 2b4b coloured by chain, front view.
PDB entry 2b4b coloured by chain, side view.
PDB entry 2b4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target.
Bewley MC, Graziano V, Jiang J, Matz E, Studier FW, Pegg AE, Coleman CS, Flanagan JM
Proc Natl Acad Sci U S A 103 2063-8 (2006)
PMID: 16455797

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diamine acetyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 20.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21673 (Residues: 1-171; Coverage: 100%)
Gene names: SAT, SAT1
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand COA 2 x COA
1 bound ligand:
Ligand B33 1 x B33
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43
Unit cell:
a: 73.809Å b: 73.809Å c: 64.083Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Targeting polyamine metabolism and function in cancer and other hyperproliferative diseases.
Casero et al. (2007)
4 more

5 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
4 more