2b4c

X-ray diffraction
3.3Å resolution

Crystal structure of HIV-1 JR-FL gp120 core protein containing the third variable region (V3) complexed with CD4 and the X5 antibody

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero hexadecamer
PDBe Complex ID:
PDB-CPX-208505 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 344 amino acids
Theoretical weight: 38.45 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q75760 (Residues: 81-483; Coverage: 40%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chain: C
Molecule details ›
Chain: C
Length: 181 amino acids
Theoretical weight: 20.13 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01730 (Residues: 26-206; Coverage: 42%)
Gene name: CD4
Sequence domains:
Structure domains: Immunoglobulins
anti-HIV-1 gp120 immunoglobulin X5 light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
anti-HIV-1 gp120 immunoglobulin X5 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 24.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P622
Unit cell:
a: 225.993Å b: 225.993Å c: 97.968Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.319 0.319 0.349
Expression systems:
  • Drosophila melanogaster
  • Cricetulus griseus
  • Escherichia coli