› 2b5i

X-ray diffraction
2.3Å resolution

cytokine receptor complex

Released:
DOI: 10.2210/pdb2b5i/pdb
PDB entry 2b5i coloured by chain, front view.
PDB entry 2b5i coloured by chain, side view.
PDB entry 2b5i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors.
Wang X, Rickert M, Garcia KC
Science 310 1159-63 (2005)
PMID: 16293754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-134426 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Interleukin-2 Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 15.44 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P60568 (Residues: 21-153; Coverage: 100%)
Gene name: IL2
Sequence domains: Interleukin 2
Structure domains: Growth Hormone; Chain: A;
Interleukin-2 receptor subunit beta Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P14784 (Residues: 27-240; Coverage: 41%)
Gene names: IL15RB, IL2RB
Sequence domains: Interleukin-2 receptor subunit beta N-terminal domain 1
Structure domains: Immunoglobulins
Cytokine receptor common subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 199 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P31785 (Residues: 56-254; Coverage: 57%)
Gene name: IL2RG
Sequence domains:
Structure domains: Immunoglobulins
Interleukin-2 receptor subunit alpha Chain: D
Molecule details ›
Chain: D
Length: 217 amino acids
Theoretical weight: 24.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01589 (Residues: 22-238; Coverage: 87%)
Gene name: IL2RA
Sequence domains: Sushi repeat (SCR repeat)
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 113.913Å b: 87.709Å c: 130.177Å
α: 90° β: 116.32° γ: 90°
R-values:
R R work R free
0.225 0.223 0.269
Expression system: Trichoplusia ni

Quick links

Citations

65 review citations

The role of interleukin-2 during homeostasis and activation of the immune system.
Boyman et al. (2012)
64 more

46 mentions without citation

A tug-of-war between the host and the pathogen generates strategic hotspots for the development of novel therapeutic interventions against infectious diseases.
Rana et al. (2015)
45 more