› 2b8y

X-ray diffraction
1.8Å resolution

Crystal structure of the interleukin-4 variant T13DF82D

Released:
DOI: 10.2210/pdb2b8y/pdb
PDB entry 2b8y coloured by chain, front view.
PDB entry 2b8y coloured by chain, side view.
PDB entry 2b8y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor.
Kraich M, Klein M, Patiño E, Harrer H, Nickel J, Sebald W, Mueller TD
BMC Biol 4 13 (2006)
PMID: 16640778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-4 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05112 (Residues: 25-153; Coverage: 100%)
Gene name: IL4
Sequence domains: Interleukin 4
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 91.032Å b: 91.032Å c: 46.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.241
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fundamental aspects of protein-protein association kinetics.
Schreiber et al. (2009)
16 more

1 mention without citation

A modular interface of IL-4 allows for scalable affinity without affecting specificity for the IL-4 receptor.
Kraich et al. (2006)