› 2bba

X-ray diffraction
1.65Å resolution

Crystal Structure and Thermodynamic Characterization of the EphB4 Receptor in Complex with an ephrin-B2 Antagonist Peptide Reveals the Determinants for Receptor Specificity.

Released:
DOI: 10.2210/pdb2bba/pdb
PDB entry 2bba coloured by chain, front view.
PDB entry 2bba coloured by chain, side view.
PDB entry 2bba coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and thermodynamic characterization of the EphB4/Ephrin-B2 antagonist peptide complex reveals the determinants for receptor specificity.
Chrencik JE, Brooun A, Recht MI, Kraus ML, Koolpe M, Kolatkar AR, Bruce RH, Martiny-Baron G, Widmer H, Pasquale EB, Kuhn P
Structure 14 321-30 (2006)
PMID: 16472751

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157091 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ephrin type-B receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 21.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54760 (Residues: 17-196; Coverage: 19%)
Gene names: EPHB4, HTK, MYK1, TYRO11
Sequence domains: Ephrin receptor ligand binding domain
Structure domains: Galactose-binding domain-like
Agonist peptide Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.71 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 60.972Å b: 60.972Å c: 151.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.174 0.191
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

21 review citations

Eph receptors and ephrins in cancer: bidirectional signalling and beyond.
Pasquale EB. (2010)
20 more

16 mentions without citation

Targeting the Eph System with Peptides and Peptide Conjugates.
Riedl et al. (2015)
15 more