› 2be6

X-ray diffraction
2Å resolution

2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex

Released:
DOI: 10.2210/pdb2be6/pdb
PDB entry 2be6 coloured by chain, front view.
PDB entry 2be6 coloured by chain, side view.
PDB entry 2be6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex.
Van Petegem F, Chatelain FC, Minor DL
Nat Struct Mol Biol 12 1108-15 (2005)
PMID: 16299511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-143947 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 150 amino acids
Theoretical weight: 16.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Voltage-dependent L-type calcium channel subunit alpha-1C Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 37 amino acids
Theoretical weight: 4.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13936 (Residues: 1659-1692; Coverage: 2%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Ligands and Environments

2 bound ligands:
Ligand CA 12 x CA
Ligand NI 3 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 84.731Å b: 37.241Å c: 86.86Å
α: 90° β: 97.77° γ: 90°
R-values:
R R work R free
0.205 0.202 0.255
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Neuronal voltage-gated calcium channels: structure, function, and dysfunction.
Simms et al. (2014)
36 more

34 mentions without citation

Calmodulin regulation (calmodulation) of voltage-gated calcium channels.
Ben-Johny et al. (2014)
33 more