› 2bij

X-ray diffraction
2.05Å resolution

Crystal structure of the human protein tyrosine phosphatase PTPN5 (STEP, striatum enriched enriched Phosphatase)

Released:
DOI: 10.2210/pdb2bij/pdb
PDB entry 2bij coloured by chain, front view.
PDB entry 2bij coloured by chain, side view.
PDB entry 2bij coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures and inhibitor identification for PTPN5, PTPRR and PTPN7: a family of human MAPK-specific protein tyrosine phosphatases.
Eswaran J, von Kries JP, Marsden B, Longman E, Debreczeni JE, Ugochukwu E, Turnbull A, Lee WH, Knapp S, Barr AJ
Biochem J 395 483-91 (2006)
PMID: 16441242

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 5 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54829 (Residues: 277-563; Coverage: 51%)
Gene name: PTPN5
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.808Å b: 64.318Å c: 101.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.264
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Therapeutic implications for striatal-enriched protein tyrosine phosphatase (STEP) in neuropsychiatric disorders.
Goebel-Goody et al. (2012)
11 more

6 mentions without citation

Large-scale structural analysis of the classical human protein tyrosine phosphatome.
Barr et al. (2009)
5 more