› 2bmd

X-ray diffraction
1.8Å resolution

high resolution structure of GDP-bound human Rab4a

Released:
DOI: 10.2210/pdb2bmd/pdb
PDB entry 2bmd coloured by chain, front view.
PDB entry 2bmd coloured by chain, side view.
PDB entry 2bmd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structures of human Rab4a in its active and inactive conformations.
Huber SK, Scheidig AJ
FEBS Lett 579 2821-9 (2005)
PMID: 15907487

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-4A Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20338 (Residues: 6-189; Coverage: 84%)
Gene names: RAB4, RAB4A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 71.14Å b: 63.75Å c: 38.28Å
α: 90° β: 100.03° γ: 90°
R-values:
R R work R free
0.162 0.16 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
2 more

2 mentions without citation

Detection of trans-cis flips and peptide-plane flips in protein structures.
Touw et al. (2015)
1 more