› 2bnu

X-ray diffraction
1.4Å resolution

Structural and kinetic basis for heightened immunogenicity of T cell vaccines

Released:
DOI: 10.2210/pdb2bnu/pdb
PDB entry 2bnu coloured by chain, front view.
PDB entry 2bnu coloured by chain, side view.
PDB entry 2bnu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic basis for heightened immunogenicity of T cell vaccines.
Chen JL, Stewart-Jones G, Bossi G, Lissin NM, Wooldridge L, Choi EM, Held G, Dunbar PR, Esnouf RM, Sami M, Boulter JM, Rizkallah P, Renner C, Sewell A, van der Merwe PA, Jakobsen BK, Griffiths G, Jones EY, Cerundolo V
J Exp Med 201 1243-55 (2005)
PMID: 15837811

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100940 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T cell receptor alpha chain constant; T cell receptor alpha variable 21 Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0B4J279 (Residues: 21-112; Coverage: 99%)
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC, TRAV21
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1; T cell receptor beta variable 6-5 Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 27.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0K0K1A5 (Residues: 22-114; Coverage: 100%)
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene names: TRBC1, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 42.991Å b: 60.295Å c: 82.641Å
α: 90° β: 89.54° γ: 90°
R-values:
R R work R free
0.245 0.245 not available
Expression system: Escherichia coli BL21

Quick links

Citations

34 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
33 more

7 mentions without citation

Conformational changes and flexibility in T-cell receptor recognition of peptide-MHC complexes.
Armstrong et al. (2008)
6 more