› 2bq8

X-ray diffraction
2.2Å resolution

Crystal structure of human purple acid phosphatase with an inhibitory conformation of the repression loop

Released:
DOI: 10.2210/pdb2bq8/pdb
PDB entry 2bq8 coloured by chain, front view.
PDB entry 2bq8 coloured by chain, side view.
PDB entry 2bq8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop.
Sträter N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM
J Mol Biol 351 233-46 (2005)
PMID: 15993892

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tartrate-resistant acid phosphatase type 5 Chain: X
Molecule details ›
Chain: X
Length: 304 amino acids
Theoretical weight: 34.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13686 (Residues: 22-325; Coverage: 100%)
Gene name: ACP5
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand FE2 2 x FE2
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P6122
Unit cell:
a: 94.756Å b: 94.756Å c: 144.007Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.211
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Metallophosphoesterases: structural fidelity with functional promiscuity.
Matange et al. (2015)
5 more

2 mentions without citation

Tartrate-resistant acid phosphatase deficiency causes a bone dysplasia with autoimmunity and a type I interferon expression signature.
Briggs et al. (2011)
1 more