› 2bzz

X-ray diffraction
0.98Å resolution

Crystal Structures of Eosinophil-derived Neurotoxin in Complex with the Inhibitors 5'-ATP, Ap3A, Ap4A and Ap5A

Released:
DOI: 10.2210/pdb2bzz/pdb
PDB entry 2bzz coloured by chain, front view.
PDB entry 2bzz coloured by chain, side view.
PDB entry 2bzz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of eosinophil-derived neurotoxin (EDN) in complex with the inhibitors 5'-ATP, Ap3A, Ap4A, and Ap5A.
Baker MD, Holloway DE, Swaminathan GJ, Acharya KR
Biochemistry 45 416-26 (2006)
PMID: 16401072

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-secretory ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10153 (Residues: 27-161; Coverage: 100%)
Gene names: EDN, RNASE2, RNS2
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand AP5 1 x AP5
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P212121
Unit cell:
a: 53.036Å b: 56.965Å c: 42.259Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

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Citations

3 review citations

Re-evaluation of Diadenosine Tetraphosphate (Ap4A) From a Stress Metabolite to Bona Fide Secondary Messenger.
Ferguson et al. (2020)
2 more

1 mention without citation

Human antimicrobial peptides and proteins.
Wang G. (2014)