› 2c60

X-ray diffraction
1.25Å resolution

crystal structure of human mitogen-activated protein kinase kinase kinase 3 isoform 2 phox domain at 1.25 A resolution

Released:
DOI: 10.2210/pdb2c60/pdb
PDB entry 2c60 coloured by chain, front view.
PDB entry 2c60 coloured by chain, side view.
PDB entry 2c60 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Debreczeni JE, Salah E, Papagrigoriou E, Burgess N, von Delft F, Gileadi O, Sundstrom M, Edwards A, Arrowsmith C, Weigelt J, Knapp S

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 3 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 13.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99759 (Residues: 27-124; Coverage: 16%)
Gene names: MAP3K3, MAPKKK3, MEKK3
Sequence domains: PB1 domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 82.8Å b: 42.3Å c: 30.6Å
α: 90° β: 106.6° γ: 90°
R-values:
R R work R free
0.166 0.165 0.182
Expression system: Escherichia coli BL21(DE3)

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