› 2c74

X-ray diffraction
2.7Å resolution

14-3-3 Protein Eta (Human) Complexed to Peptide

Released:
DOI: 10.2210/pdb2c74/pdb
PDB entry 2c74 coloured by chain, front view.
PDB entry 2c74 coloured by chain, side view.
PDB entry 2c74 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for protein-protein interactions in the 14-3-3 protein family.
Yang X, Lee WH, Sobott F, Papagrigoriou E, Robinson CV, Grossmann JG, Sundström M, Doyle DA, Elkins JM
Proc Natl Acad Sci U S A 103 17237-42 (2006)
PMID: 17085597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162659 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein eta Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 28.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04917 (Residues: 1-246; Coverage: 100%)
Gene names: YWHA1, YWHAH
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
CONSENSUS PEPTIDE MODE 1 FOR 14-3-3 PROTEINS Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 7 amino acids
Theoretical weight: 953 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 75.731Å b: 75.36Å c: 113.529Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.291
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

26 review citations

Recent advances in the development of protein-protein interactions modulators: mechanisms and clinical trials.
Lu et al. (2020)
25 more

5 mentions without citation

Structural basis for protein-protein interactions in the 14-3-3 protein family.
Yang et al. (2006)
4 more