› 2c9o

X-ray diffraction
2.2Å resolution

3D Structure of the human RuvB-like helicase RuvBL1

Released:
DOI: 10.2210/pdb2c9o/pdb
PDB entry 2c9o coloured by chain, front view.
PDB entry 2c9o coloured by chain, side view.
PDB entry 2c9o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human AAA+ protein RuvBL1.
Matias PM, Gorynia S, Donner P, Carrondo MA
J Biol Chem 281 38918-29 (2006)
PMID: 17060327

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 456 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6
Unit cell:
a: 207.08Å b: 207.08Å c: 60.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
23 more

23 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
22 more