› 2ceu

X-ray diffraction
1.8Å resolution

Despentapeptide insulin in acetic acid (pH 2)

Released:
DOI: 10.2210/pdb2ceu/pdb
PDB entry 2ceu coloured by chain, front view.
PDB entry 2ceu coloured by chain, side view.
PDB entry 2ceu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
I222 crystal form of despentapeptide (B26-B30) insulin provides new insights into the properties of monomeric insulin.
Whittingham JL, Youshang Z, Záková L, Dodson EJ, Turkenburg JP, Brange J, Dodson GG
Acta Crystallogr D Biol Crystallogr 62 505-11 (2006)
PMID: 16627943

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 25 amino acids
Theoretical weight: 2.84 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-49; Coverage: 29%)
Gene name: INS

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I222
Unit cell:
a: 58.152Å b: 58.174Å c: 54.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.211
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 citation in other articles

Role of Proinsulin Self-Association in Mutant INS Gene-Induced Diabetes of Youth.
Sun et al. (2020)
1 more