› 2cfy

X-ray diffraction
2.7Å resolution

Crystal structure of human thioredoxin reductase 1

Released:
DOI: 10.2210/pdb2cfy/pdb
PDB entry 2cfy coloured by chain, front view.
PDB entry 2cfy coloured by chain, side view.
PDB entry 2cfy coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Debreczeni JE, Johansson C, Kavanagh K, Savitsky P, Sundstrom M, Arrowsmith C, Weigelt J, Edwards A, von Delft F, Oppermann U

Function and Biology Details

Reactions catalysed: 
NADPH + H(2)O(2) = NADP(+) + 2 H(2)O
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase 1, cytoplasmic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 521 amino acids
Theoretical weight: 57.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16881 (Residues: 150-649; Coverage: 77%)
Gene names: GRIM12, KDRF, TXNRD1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 6 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P2
Unit cell:
a: 89.756Å b: 149.651Å c: 146.789Å
α: 90° β: 91.96° γ: 90°
R-values:
R R work R free
0.205 0.203 0.248
Expression system: Escherichia coli BL21(DE3)

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