› 2ci8

X-ray diffraction
1.8Å resolution

sh2 domain of human nck1 adaptor protein - uncomplexed

Released:
DOI: 10.2210/pdb2ci8/pdb
PDB entry 2ci8 coloured by chain, front view.
PDB entry 2ci8 coloured by chain, side view.
PDB entry 2ci8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
Frese S, Schubert WD, Findeis AC, Marquardt T, Roske YS, Stradal TE, Heinz DW
J Biol Chem 281 18236-45 (2006)
PMID: 16636066

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SH2/SH3 adapter protein NCK1 Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16333 (Residues: 281-377; Coverage: 26%)
Gene names: NCK, NCK1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C222
Unit cell:
a: 62.854Å b: 82.484Å c: 44.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.274
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Functions and regulation of circular dorsal ruffles.
Hoon et al. (2012)
7 more

3 mentions without citation

Linking in domain-swapped protein dimers.
Baiesi et al. (2016)
2 more