› 2cjz

X-ray diffraction
1.7Å resolution

crystal structure of the c472s mutant of human protein tyrosine phosphatase ptpn5 (step, striatum enriched phosphatase) in complex with phosphotyrosine

Released:
DOI: 10.2210/pdb2cjz/pdb
PDB entry 2cjz coloured by chain, front view.
PDB entry 2cjz coloured by chain, side view.
PDB entry 2cjz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Large-scale structural analysis of the classical human protein tyrosine phosphatome.
Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Müller S, Knapp S
Cell 136 352-63 (2009)
PMID: 19167335

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 5 Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54829 (Residues: 277-563; Coverage: 51%)
Gene name: PTPN5
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand PTR 1 x PTR
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 52.342Å b: 64.348Å c: 100.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

59 review citations

Inside the human cancer tyrosine phosphatome.
Julien et al. (2011)
58 more

10 mentions without citation

Regulation of Three Key Kinases of Brassinosteroid Signaling Pathway.
Mao et al. (2020)
9 more