› 2cko

X-ray diffraction
2.15Å resolution

Crystal structure of Human Choline Kinase alpha 2

Released:
DOI: 10.2210/pdb2cko/pdb
PDB entry 2cko coloured by chain, front view.
PDB entry 2cko coloured by chain, side view.
PDB entry 2cko coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine.
Malito E, Sekulic N, Too WC, Konrad M, Lavie A
J Mol Biol 364 136-51 (2006)
PMID: 17007874

Function and Biology Details

Reactions catalysed: 
ATP + choline = ADP + phosphocholine
ATP + ethanolamine = ADP + O-phosphoethanolamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline kinase alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 45.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35790 (Residues: 50-457; Coverage: 85%)
Gene names: CHK, CHKA, CKI
Sequence domains: Choline/ethanolamine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DTT 2 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 56.18Å b: 122.82Å c: 132.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The genomic enzymology of antibiotic resistance.
Morar et al. (2010)
4 more

8 mentions without citation

Choline Kinase: An Unexpected Journey for a Precision Medicine Strategy in Human Diseases.
Lacal et al. (2021)
7 more