› 2cl7

X-ray diffraction
1.25Å resolution

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GTP

Released:
DOI: 10.2210/pdb2cl7/pdb
PDB entry 2cl7 coloured by chain, ensemble of 2 models, front view.
PDB entry 2cl7 coloured by chain, ensemble of 2 models, side view.
PDB entry 2cl7 coloured by chain, ensemble of 2 models, top view.
Source organism: Homo sapiens
Primary publication:
A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.
Klink BU, Goody RS, Scheidig AJ
Biophys J 91 981-92 (2006)
PMID: 16698776

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: X
Molecule details ›
Chain: X
Length: 166 amino acids
Theoretical weight: 18.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand XY2 1 x XY2
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41
Unit cell:
a: 69.28Å b: 69.28Å c: 35.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.17
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal Fluorides as Analogues for Studies on Phosphoryl Transfer Enzymes.
Jin et al. (2017)
1 more

9 mentions without citation

Ras conformational switching: simulating nucleotide-dependent conformational transitions with accelerated molecular dynamics.
Grant et al. (2009)
8 more