› 2clq

X-ray diffraction
2.3Å resolution

Structure of mitogen-activated protein kinase kinase kinase 5

Released:
DOI: 10.2210/pdb2clq/pdb
PDB entry 2clq coloured by chain, front view.
PDB entry 2clq coloured by chain, side view.
PDB entry 2clq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional characterization of the human protein kinase ASK1.
Bunkoczi G, Salah E, Filippakopoulos P, Fedorov O, Müller S, Sobott F, Parker SA, Zhang H, Min W, Turk BE, Knapp S
Structure 15 1215-26 (2007)
PMID: 17937911

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99683 (Residues: 659-951; Coverage: 21%)
Gene names: ASK1, MAP3K5, MAPKKK5, MEKK5
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 2 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 78.606Å b: 78.606Å c: 423.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Thioredoxin and thioredoxin target proteins: from molecular mechanisms to functional significance.
Lee et al. (2013)
14 more

17 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
16 more