› 2cn1

X-ray diffraction
2.67Å resolution

Crystal structure of Human Cytosolic 5'-Nucleotidase III (NT5C3)

Released:
DOI: 10.2210/pdb2cn1/pdb
PDB entry 2cn1 coloured by chain, front view.
PDB entry 2cn1 coloured by chain, side view.
PDB entry 2cn1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.
Walldén K, Stenmark P, Nyman T, Flodin S, Gräslund S, Loppnau P, Bianchi V, Nordlund P
J Biol Chem 282 17828-36 (2007)
PMID: 17405878

Function and Biology Details

Reactions catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
CMP + H(2)O = cytidine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic 5'-nucleotidase 3A Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0P0 (Residues: 64-336; Coverage: 81%)
Gene names: HSPC233, NT5C3, NT5C3A, P5N1, UMPH1
Sequence domains: Pyrimidine 5'-nucleotidase (UMPH-1)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 86.389Å b: 100.951Å c: 76.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Therapeutic potentials of ecto-nucleoside triphosphate diphosphohydrolase, ecto-nucleotide pyrophosphatase/phosphodiesterase, ecto-5'-nucleotidase, and alkaline phosphatase inhibitors.
al-Rashida et al. (2014)
4 more

3 mentions without citation

Cytosolic 5'-nucleotidase III (NT5C3): gene sequence variation and functional genomics.
Aksoy et al. (2009)
2 more