› 2cst

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE CLOSED FORM OF CHICKEN CYTOSOLIC ASPARTATE AMINOTRANSFERASE AT 1.9 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb2cst/pdb
PDB entry 2cst coloured by chain, front view.
PDB entry 2cst coloured by chain, side view.
PDB entry 2cst coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystal structure of the closed form of chicken cytosolic aspartate aminotransferase at 1.9 A resolution.
Malashkevich VN, Strokopytov BV, Borisov VV, Dauter Z, Wilson KS, Torchinsky YM
J Mol Biol 247 111-24 (1995)
PMID: 7897655

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 45.86 KDa
Source organism: Gallus gallus
Expression system: unidentified
UniProt:
  • Canonical: P00504 (Residues: 2-412; Coverage: 100%)
Gene name: GOT1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand MAE 2 x MAE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 56.4Å b: 126Å c: 124.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 not available not available
Expression system: unidentified

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Citations

6 review citations

Structure, evolution and action of vitamin B6-dependent enzymes.
Jansonius JN. (1998)
5 more

9 mentions without citation

The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.
Janssen et al. (2010)
8 more