› 2d4q

X-ray diffraction
2.3Å resolution

Crystal structure of the Sec-PH domain of the human neurofibromatosis type 1 protein

Released:
DOI: 10.2210/pdb2d4q/pdb
PDB entry 2d4q coloured by chain, front view.
PDB entry 2d4q coloured by chain, side view.
PDB entry 2d4q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel bipartite phospholipid-binding module in the neurofibromatosis type 1 protein.
D'Angelo I, Welti S, Bonneau F, Scheffzek K
EMBO Rep 7 174-9 (2006)
PMID: 16397625

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurofibromin Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21359 (Residues: 1581-1837; Coverage: 9%)
Gene name: NF1
Sequence domains: Divergent CRAL/TRIO domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand OXN 2 x OXN
Ligand POP 2 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 113.5Å b: 113.5Å c: 125.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.33 0.24 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

GEFs and GAPs: critical elements in the control of small G proteins.
Bos et al. (2007)
31 more

8 mentions without citation

High Incidence of Noonan Syndrome Features Including Short Stature and Pulmonic Stenosis in Patients carrying NF1 Missense Mutations Affecting p.Arg1809: Genotype-Phenotype Correlation.
Rojnueangnit et al. (2015)
7 more