› 2ddy

Solution NMR

Solution Structure of Matrilysin (MMP-7) Complexed to Constraint Conformational Sulfonamide Inhibitor

Released:
DOI: 10.2210/pdb2ddy/pdb
PDB entry 2ddy coloured by chain, ensemble of 25 models, front view.
PDB entry 2ddy coloured by chain, ensemble of 25 models, side view.
PDB entry 2ddy coloured by chain, ensemble of 25 models, top view.
Source organism: Homo sapiens
Entry authors: Zheng XH, Ou L

Function and Biology Details

Reaction catalysed: 
Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrilysin Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 19.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09237 (Residues: 95-267; Coverage: 69%)
Gene names: MMP7, MPSL1, PUMP1
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
Ligand MDW 1 x MDW
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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