› 2dgn

X-ray diffraction
2.4Å resolution

Mouse Muscle Adenylosuccinate Synthetase partially ligated complex with GTP, 2'-deoxy-IMP

Released:
DOI: 10.2210/pdb2dgn/pdb
PDB entry 2dgn coloured by chain, front view.
PDB entry 2dgn coloured by chain, side view.
PDB entry 2dgn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cavitation as a mechanism of substrate discrimination by adenylosuccinate synthetases.
Iancu CV, Zhou Y, Borza T, Fromm HJ, Honzatko RB
Biochemistry 45 11703-11 (2006)
PMID: 16981730

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase isozyme 1 Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 50.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28650 (Residues: 1-457; Coverage: 100%)
Gene names: Adss1, Adssl1
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand DOI 1 x DOI
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 70.112Å b: 70.112Å c: 199.033Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Mechanisms supporting aminoadenine-based viral DNA genomes.
Kaminski PA. (2021)
3 other articles

2 mentions without citation

Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA.
Czernecki et al. (2021)
1 more