› 2dq5

Solution NMR

solution structure of the Mid1 B Box2 Chc(D/C)C2H2 Zinc-Binding Domain: insights into an evolutionary conserved ring fold

Released:
DOI: 10.2210/pdb2dq5/pdb
PDB entry 2dq5 coloured by chain, ensemble of 16 models, front view.
PDB entry 2dq5 coloured by chain, ensemble of 16 models, side view.
PDB entry 2dq5 coloured by chain, ensemble of 16 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the MID1 B-box2 CHC(D/C)C(2)H(2) zinc-binding domain: insights into an evolutionarily conserved RING fold.
Massiah MA, Matts JA, Short KM, Simmons BN, Singireddy S, Yi Z, Cox TC
J Mol Biol 369 1-10 (2007)
PMID: 17428496

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Midline-1 Chain: A
Molecule details ›
Chain: A
Length: 47 amino acids
Theoretical weight: 5.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15344 (Residues: 168-214; Coverage: 7%)
Gene names: FXY, MID1, RNF59, TRIM18, XPRF
Sequence domains: B-box zinc finger
Structure domains: CD2-Gal4

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, low target function
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The control of viral infection by tripartite motif proteins and cyclophilin A.
Towers GJ. (2007)
11 more

2 mentions without citation

Solution NMR structure of the TRIM21 B-box2 and identification of residues involved in its interaction with the RING domain.
Wallenhammar et al. (2017)
1 more