› 2dsr

X-ray diffraction
2.1Å resolution

Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins

Released:
DOI: 10.2210/pdb2dsr/pdb
PDB entry 2dsr coloured by chain, front view.
PDB entry 2dsr coloured by chain, side view.
PDB entry 2dsr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the inhibition of insulin-like growth factors by insulin-like growth factor-binding proteins.
Sitar T, Popowicz GM, Siwanowicz I, Huber R, Holak TA
Proc Natl Acad Sci U S A 103 13028-33 (2006)
PMID: 16924115

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138316 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin-like growth factor-binding protein 4 Chain: G
Molecule details ›
Chain: G
Length: 82 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 172-253; Coverage: 35%)
Gene names: IBP4, IGFBP4
Sequence domains: Thyroglobulin type-1 repeat
Structure domains: Thyroglobulin type-1
Insulin-like growth factor-binding protein 4 Chain: B
Molecule details ›
Chain: B
Length: 80 amino acids
Theoretical weight: 8.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22692 (Residues: 24-103; Coverage: 34%)
Gene names: IBP4, IGFBP4
Sequence domains: Insulin-like growth factor binding protein
Structure domains: Omega-AgatoxinV
Insulin-like growth factor I Chain: I
Molecule details ›
Chain: I
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05019 (Residues: 49-118; Coverage: 40%)
Gene names: IBP1, IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 74.4Å b: 50.25Å c: 64.3Å
α: 90° β: 115.3° γ: 90°
R-values:
R R work R free
0.202 0.199 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

IGF binding proteins in cancer: mechanistic and clinical insights.
Baxter RC. (2014)
26 more

13 mentions without citation

Defining the pathway to insulin-like growth factor system targeting in cancer.
Rosenzweig et al. (2010)
12 more