› 2dx1

X-ray diffraction
2.36Å resolution

Crystal structure of RhoGEF protein Asef

Released:
DOI: 10.2210/pdb2dx1/pdb
PDB entry 2dx1 coloured by chain, front view.
PDB entry 2dx1 coloured by chain, side view.
PDB entry 2dx1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the rac activator, Asef, reveals its autoinhibitory mechanism.
Murayama K, Shirouzu M, Kawasaki Y, Kato-Murayama M, Hanawa-Suetsugu K, Sakamoto A, Katsura Y, Suenaga A, Toyama M, Terada T, Taiji M, Akiyama T, Yokoyama S
J. Biol. Chem. 282 4238-42 (2007)
PMID: 17190834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 4 Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.62 KDa
Source organism: Homo sapiens
Expression system: CELL FREE PROTEIN SYNTHESIS
UniProt:
  • Canonical: Q9NR80 (Residues: 143-611; Coverage: 68%)
Gene names: ARHGEF4, KIAA1112
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 100.932Å b: 79.818Å c: 68.004Å
α: 90° β: 123.25° γ: 90°
R-values:
R R work R free
0.232 0.232 0.299
Expression system: CELL FREE PROTEIN SYNTHESIS

Quick links

Citations

11 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
10 more

1 mention without citation

SH3 domain regulation of RhoGAP activity: Crosstalk between p120RasGAP and DLC1 RhoGAP.
Chau et al. (2022)