› 2e1f

X-ray diffraction
2Å resolution

Crystal structure of the HRDC Domain of Human Werner Syndrome Protein, WRN

Released:
DOI: 10.2210/pdb2e1f/pdb
PDB entry 2e1f coloured by chain, front view.
PDB entry 2e1f coloured by chain, side view.
PDB entry 2e1f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the HRDC domain of human Werner syndrome protein, WRN.
Kitano K, Yoshihara N, Hakoshima T
J Biol Chem 282 2717-28 (2007)
PMID: 17148451

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-171958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 11.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14191 (Residues: 1142-1242; Coverage: 7%)
Gene names: RECQ3, RECQL2, WRN
Sequence domains: HRDC domain
Structure domains: HRDC domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P6222
Unit cell:
a: 61.664Å b: 61.664Å c: 136.683Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.248 0.255
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Human RecQ helicases in DNA repair, recombination, and replication.
Croteau et al. (2014)
14 more

2 mentions without citation

X-ray vs. NMR structures as templates for computational protein design.
Schneider et al. (2009)
1 more