› 2e42

X-ray diffraction
1.8Å resolution

Crystal structure of C/EBPbeta Bzip homodimer V285A mutant bound to A High Affinity DNA fragment

Released:
DOI: 10.2210/pdb2e42/pdb
PDB entry 2e42 coloured by chain, front view.
PDB entry 2e42 coloured by chain, side view.
PDB entry 2e42 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tahirov TH, Inoue-Bungo T, Sato K, Shiina M, Hamada K, Ogata K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115200 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-336; Coverage: 23%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
DNA (5'-D(P*DTP*DAP*DGP*DGP*DAP*DTP*DTP*DGP*DCP*DGP*DCP*DAP*DAP*DTP*DAP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*DAP*DAP*DTP*DAP*DTP*DTP*DGP*DCP*DGP*DCP*DAP*DAP*DTP*DCP*DCP*DT)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2221
Unit cell:
a: 101.881Å b: 113.319Å c: 74.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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