› 2e8a

X-ray diffraction
1.77Å resolution

Crystal structure of the human Hsp70 ATPase domain in complex with AMP-PNP

Released:
DOI: 10.2210/pdb2e8a/pdb
PDB entry 2e8a coloured by chain, front view.
PDB entry 2e8a coloured by chain, side view.
PDB entry 2e8a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Direct inter-subdomain interactions switch between the closed and open forms of the Hsp70 nucleotide-binding domain in the nucleotide-free state.
Shida M, Arakawa A, Ishii R, Kishishita S, Takagi T, Kukimoto-Niino M, Sugano S, Tanaka A, Shirouzu M, Yokoyama S
Acta Crystallogr D Biol Crystallogr 66 223-32 (2010)
PMID: 20179333

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock 70 kDa protein 1A Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 43.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV8 (Residues: 1-388; Coverage: 61%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 45.49Å b: 62.22Å c: 143.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

HSP70 Multi-Functionality in Cancer.
Albakova et al. (2020)
1 more

12 mentions without citation

Predicting new indications for approved drugs using a proteochemometric method.
Dakshanamurthy et al. (2012)
11 more