› 2eal

X-ray diffraction
1.85Å resolution

Crystal structure of human galectin-9 N-terminal CRD in complex with Forssman pentasaccharide

Released:
DOI: 10.2210/pdb2eal/pdb
PDB entry 2eal coloured by chain, front view.
PDB entry 2eal coloured by chain, side view.
PDB entry 2eal coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the human galectin-9 N-terminal carbohydrate recognition domain reveals unexpected properties that differ from the mouse orthologue.
Nagae M, Nishi N, Nakamura-Tsuruta S, Hirabayashi J, Wakatsuki S, Kato R
J Mol Biol 375 119-35 (2008)
PMID: 18005988

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00182 (Residues: 1-148; Coverage: 42%)
Gene name: LGALS9
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, NGA, A2G
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 38.486Å b: 68.836Å c: 106.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Therapeutic potential of Galectin-9 in human disease.
Wiersma et al. (2013)
7 more

5 mentions without citation

The Structural Biology of Galectin-Ligand Recognition: Current Advances in Modeling Tools, Protein Engineering, and Inhibitor Design.
Modenutti et al. (2019)
4 more