› 2exu

X-ray diffraction
2.23Å resolution

Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain

Released:
DOI: 10.2210/pdb2exu/pdb
PDB entry 2exu coloured by chain, front view.
PDB entry 2exu coloured by chain, side view.
PDB entry 2exu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Xu F, Guo M, Fang P, Teng M, Niu L

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor SPT4 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32914 (Residues: 1-99; Coverage: 97%)
Gene names: SPT4, YGR063C
Sequence domains: Spt4/RpoE2 zinc finger
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand EOH 2 x EOH
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A, RIGAKU
Spacegroup: P4122
Unit cell:
a: 53.801Å b: 53.801Å c: 177.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.221
Expression system: Escherichia coli

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