› 2ezd

Solution NMR

SOLUTION STRUCTURE OF A COMPLEX OF THE SECOND DNA BINDING DOMAIN OF HUMAN HMG-I(Y) BOUND TO DNA DODECAMER CONTAINING THE PRDII SITE OF THE INTERFERON-BETA PROMOTER, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb2ezd/pdb
PDB entry 2ezd coloured by chain, front view.
PDB entry 2ezd coloured by chain, side view.
PDB entry 2ezd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The solution structure of an HMG-I(Y)-DNA complex defines a new architectural minor groove binding motif.
Huth JR, Bewley CA, Nissen MS, Evans JN, Reeves R, Gronenborn AM, Clore GM
Nat Struct Biol 4 657-65 (1997)
PMID: 9253416

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115227 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
High mobility group protein HMG-I/HMG-Y Chain: A
Molecule details ›
Chain: A
Length: 25 amino acids
Theoretical weight: 2.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17096 (Residues: 51-75; Coverage: 23%)
Gene names: HMGA1, HMGIY
DNA (5'-D(*GP*GP*GP*AP*AP*AP*TP*TP*CP*CP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*AP*GP*GP*AP*AP*TP*TP*TP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

40 review citations

Intrinsically disordered protein.
Dunker et al. (2001)
39 more

15 mentions without citation

Interpreting protein/DNA interactions: distinguishing specific from non-specific and electrostatic from non-electrostatic components.
Privalov et al. (2011)
14 more