› 2f1z

X-ray diffraction
3.2Å resolution

Crystal structure of HAUSP

Released:
DOI: 10.2210/pdb2f1z/pdb
PDB entry 2f1z coloured by chain, front view.
PDB entry 2f1z coloured by chain, side view.
PDB entry 2f1z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway.
Hu M, Gu L, Li M, Jeffrey PD, Gu W, Shi Y
PLoS Biol 4 e27 (2006)
PMID: 16402859

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187973 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 522 amino acids
Theoretical weight: 60.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93009 (Residues: 43-560; Coverage: 47%)
Gene names: HAUSP, USP7
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 97.625Å b: 219.858Å c: 130.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.265 0.316
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Structural biology of the tumor suppressor p53.
Joerger et al. (2008)
39 more

13 mentions without citation

USP7: Structure, substrate specificity, and inhibition.
Pozhidaeva et al. (2019)
12 more