› 2f3d

X-ray diffraction
1.83Å resolution

Mechanism of displacement of a catalytically essential loop from the active site of fructose-1,6-bisphosphatase

Released:
DOI: 10.2210/pdb2f3d/pdb
PDB entry 2f3d coloured by chain, front view.
PDB entry 2f3d coloured by chain, side view.
PDB entry 2f3d coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Mechanism of displacement of a catalytically essential loop from the active site of mammalian fructose-1,6-bisphosphatase.
Gao Y, Iancu CV, Mukind S, Choe JY, Honzatko RB
Biochemistry 52 5206-16 (2013)
PMID: 23844654

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 36.82 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00636 (Residues: 1-338; Coverage: 100%)
Gene names: FBP, FBP1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand F6P 1 x F6P
Ligand PO4 1 x PO4
Ligand ZN 3 x ZN
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I222
Unit cell:
a: 55.84Å b: 82.502Å c: 165.025Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.246
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Fructose 1,6-bisphosphatase: getting the message across.
Timson DJ. (2019)
2 more

3 mentions without citation

Crystal structures of human muscle fructose-1,6-bisphosphatase: novel quaternary states, enhanced AMP affinity, and allosteric signal transmission pathway.
Shi et al. (2013)
2 more