› 2f54

X-ray diffraction
2.7Å resolution

Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity

Released:
DOI: 10.2210/pdb2f54/pdb
PDB entry 2f54 coloured by chain, front view.
PDB entry 2f54 coloured by chain, side view.
PDB entry 2f54 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity.
Dunn SM, Rizkallah PJ, Baston E, Mahon T, Cameron B, Moysey R, Gao F, Sami M, Boulter J, Li Y, Jakobsen BK
Protein Sci 15 710-21 (2006)
PMID: 16600963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134728 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 274 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 157-165; Coverage: 5%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
T cell receptor alpha chain constant Chains: D, K
Molecule details ›
Chains: D, K
Length: 206 amino acids
Theoretical weight: 22.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-91; Coverage: 65%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, L
Molecule details ›
Chains: E, L
Length: 241 amino acids
Theoretical weight: 27.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21
Unit cell:
a: 120.02Å b: 53.592Å c: 152.831Å
α: 90° β: 96.04° γ: 90°
R-values:
R R work R free
0.225 0.221 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

T cell receptor-based cancer immunotherapy: Emerging efficacy and pathways of resistance.
Chandran et al. (2019)
16 more

8 mentions without citation

Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide-MHC without increasing apparent cross-reactivity.
Dunn et al. (2006)
7 more