› 2f60

X-ray diffraction
1.55Å resolution

Crystal Structure of the Dihydrolipoamide Dehydrogenase (E3)-Binding Domain of Human E3-Binding Protein

Released:
DOI: 10.2210/pdb2f60/pdb
PDB entry 2f60 coloured by chain, front view.
PDB entry 2f60 coloured by chain, side view.
PDB entry 2f60 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into interactions between dihydrolipoamide dehydrogenase (E3) and E3 binding protein of human pyruvate dehydrogenase complex.
Brautigam CA, Wynn RM, Chuang JL, Machius M, Tomchick DR, Chuang DT
Structure 14 611-21 (2006)
PMID: 16442803

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate dehydrogenase protein X component, mitochondrial Chain: K
Molecule details ›
Chain: K
Length: 64 amino acids
Theoretical weight: 7.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O00330 (Residues: 173-230; Coverage: 12%)
Gene names: PDHX, PDX1
Sequence domains: e3 binding domain
Structure domains: E3-binding domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 36.845Å b: 36.845Å c: 191.542Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

The pyruvate dehydrogenase complexes: structure-based function and regulation.
Patel et al. (2014)
13 more

7 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
6 more