› 2ff0

Solution NMR

Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter

Released:
DOI: 10.2210/pdb2ff0/pdb
PDB entry 2ff0 coloured by chain, ensemble of 16 models, front view.
PDB entry 2ff0 coloured by chain, ensemble of 16 models, side view.
PDB entry 2ff0 coloured by chain, ensemble of 16 models, top view.
Source organism: Mus musculus
Primary publication:
Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability.
Little TH, Zhang Y, Matulis CK, Weck J, Zhang Z, Ramachandran A, Mayo KE, Radhakrishnan I
Mol Endocrinol 20 831-43 (2006)
PMID: 16339274

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115253 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Steroidogenic factor 1 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 11.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33242 (Residues: 10-111; Coverage: 22%)
Gene names: Ftzf1, Nr5a1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
GGCTCAGGGCCACAG Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Mus musculus
Expression system: Not provided
CTGTGGCCCTGAGCC Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.56 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Simulated annealing and cartesian molecular dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Minireview: steroidogenic factor 1: its roles in differentiation, development, and disease.
Schimmer et al. (2010)
7 more

10 mentions without citation

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
9 more