› 2ff3

X-ray diffraction
2Å resolution

Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin

Released:
DOI: 10.2210/pdb2ff3/pdb
PDB entry 2ff3 coloured by chain, front view.
PDB entry 2ff3 coloured by chain, side view.
PDB entry 2ff3 coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis of actin interaction with multiple WH2/beta-thymosin motif-containing proteins.
Aguda AH, Xue B, Irobi E, Préat T, Robinson RC
Structure 14 469-76 (2006)
PMID: 16531231

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-126256 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Gelsolin Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 52-179; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Actin nucleation-promoting factor WASL Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00401 (Residues: 451-465; Coverage: 3%)
Gene name: WASL
Actin, alpha skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 56.996Å b: 69.347Å c: 179.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.25
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The beta-thymosins: intracellular and extracellular activities of a versatile actin binding protein family.
Mannherz et al. (2009)
7 more

4 mentions without citation

Classification of intrinsically disordered regions and proteins.
van der Lee et al. (2014)
3 more