› 2ff6

X-ray diffraction
2.05Å resolution

Crystal structure of Gelsolin domain 1:ciboulot domain 2 hybrid in complex with actin

Released:
DOI: 10.2210/pdb2ff6/pdb
PDB entry 2ff6 coloured by chain, front view.
PDB entry 2ff6 coloured by chain, side view.
PDB entry 2ff6 coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis of actin interaction with multiple WH2/beta-thymosin motif-containing proteins.
Aguda AH, Xue B, Irobi E, Préat T, Robinson RC
Structure 14 469-76 (2006)
PMID: 16531231

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-139015 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 52-179; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Ciboulot, isoform C Chain: H
Molecule details ›
Chain: H
Length: 26 amino acids
Theoretical weight: 3.09 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IRS7 (Residues: 72-97; Coverage: 27%)
Gene names: CG4944, Dmel\CG4944, Dmel_CG4944, EG0007-11, EG:EG0007.11, cib, cib-RC
Sequence domains: Thymosin beta-4 family
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 56.221Å b: 69.373Å c: 79.65Å
α: 90° β: 95.1° γ: 90°
R-values:
R R work R free
0.204 0.202 0.237
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The beta-thymosins: intracellular and extracellular activities of a versatile actin binding protein family.
Mannherz et al. (2009)
7 more

3 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
2 more